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Product Overview Product Name Recombinant DNA/RNA Nuclease Target Symbol NucA Alternative Names Nuclease A, NucA Accession Number P13717.2 Expression System E. coli. Molecular Weight 28.9 kDa (monomer, predicted) Sequence Met1-Asn266 Purity >98-99% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining. Activity Determined by its ability to cleave all single or double stranded DNA and RNA similar to the universal nuclease Benzonase®. The specific activity is typically >1.5×106 Unit per mg. Formulation PBS, pH 7.4, with 50% Glycerol and 2 mM MgCl2 Endotoxin Level <0.001 EU per 1000 Units (< 1 EU/1000KU) of the protein as determined by the LAL method. Shipping Shipped on blue ice. Store immediately at -20°C upon receipt. Stability & Storage 12 months from date of receipt at -20°C. Do not store at -80°C. Target Function Serratia marcescens Nuclease A (NucA) is a secreted, magnesium-dependent endonuclease that indiscriminately degrades both DNA and RNA, whether single- or double-stranded, linear, circular, or chromosomal, plasmid or viral, into short 2–5-base oligonucleotides. Recombinant NucA, such as Nuclease, has applications in bio-processing to meet guidelines for host cell DNA and viral DNA clearance, lowering lysate viscosity, improving clarification and chromatography, and cleaning up nucleic-acid contamination in cell-free assays. Cellular Localization Secreted enzyme